LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2363..2459) /label=attL2 /note="attL2 (c)" misc_signal complement(2474..2493) /note="T7 Promoter/priming site (c)" primer_bind 2501..2517 /note="M13 Reverse priming site" gene 2630..3439 /note="Kanamycin resistance gene" rep_origin 3560..4233 /note="pUC origin" vector join(2371..4236,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2356 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:71796 IMAGE:30335722" /tissue_type="Placenta, pre-eclamptic" /clone_lib="NIH_MGC_148" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 692..2356 /gene="C3orf39" /gene_synonym=AGO61 /gene_synonym=FLJ14566 /db_xref="GeneID:84892" /db_xref="HGNC:25902" CDS 692..2356 /dnas_title="chromosome 3 open reading frame 39" /gene="C3orf39" /gene_synonym=AGO61 /gene_synonym=FLJ14566 /codon_start=1 /product="chromosome 3 open reading frame 39" /protein_id="AAH60861.1" /db_xref="GI:38173820" /db_xref="GeneID:84892" /db_xref="HGNC:25902" /translation="MHLSAVFNALLVSVLAAVLWKHVRLREHAATLEEELALSRQATE PAPALRIDYPKALQILMEGGTHMVCTGRTHTDRICRFKWLCYSNEAEEFIFFHGNTSV MLPNLGSRRFQPALLDLSTVEDHNTQYFNFVELPAAALRFMPKPVFVPDVALIANRFN PDNLMHVFHDDLLPLFYTLRQFPGLAHEARLFFMEGWGEGAHFDLYKLLSPKQPLLRA QLKTLGRLLCFSHAFVGLSKITTWYQYGFVQPQGPKANILVSGNEIRQFARFMTEKLN VSHTGVPLGEEYILVFSRTQNRLILNEAELLLALAQEFQMKTVTVSLEDHTFADVVRL VSNASMLVSMHGAQLVTTLFLPRGATVVELFPYAVNPDHYTPYKTLAMLPGMDLQYVA WRNMMPENTVTHPERPWDQGGITHLDRAEQARILQSREVPRHLCCRNPEWLFRIYQDT KVDIPSLIQTIRRVVKGRPGPRKQKWTVGLYPGKVREARCQASVHGASEARLTVSWQI PWNLKYLKVREVKYEVWLQEQGENTYVPYILALQNHTFTENIKPFTTYLVWVRCIFNK ILLGPFADVLVCNT" misc_feature 692..2356 /note="AGO61 coding region" misc_difference 1981..1981 /gene="C3orf39" /gene_synonym=AGO61 /gene_synonym=FLJ14566 /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccgtgagcat gcagccacac tggaggagga 721 gctggccctc agccgacagg ccacagagcc agccccagca ctgaggatcg actacccgaa 781 ggcactgcag atcctgatgg agggcggcac acacatggtg tgcacgggcc gcacgcacac 841 agaccgcatc tgccgcttca agtggctctg ctactccaac gaggctgagg agttcatctt 901 cttccatggc aacacctctg tcatgctgcc caacctgggc tcccggcgct tccagccagc 961 cctgctcgac ctatccaccg tggaggacca caacactcag tacttcaact tcgtggagct 1021 gcctgctgct gccctgcgct tcatgcccaa gccggtgttc gtgccagacg tggccctcat 1081 cgccaaccgc ttcaaccccg acaacctcat gcacgtcttt catgacgacc tgctgccact 1141 cttctacacc ctgcggcagt ttcccggcct ggcccacgag gcacggctct tcttcatgga 1201 gggctggggc gagggtgcac acttcgacct ctacaagctg ctcagcccca agcagcctct 1261 cctgcgggca cagctgaaga ccctgggccg gctgctgtgc ttctcccatg cttttgtggg 1321 cctctccaag atcactacct ggtaccagta tggctttgtg cagccccagg gcccgaaggc 1381 caacatcctc gtctcaggca atgagatccg gcagtttgca cggttcatga cagaaaagct 1441 gaacgtgagc cacacaggag tccccctagg cgaggagtac attctggtct ttagccgaac 1501 ccagaacaga ctcattctga atgaggcaga gctgctgctg gcactggccc aggagttcca 1561 gatgaagaca gtgacagtgt ccctggagga ccacaccttt gctgatgtcg tgcggctggt 1621 cagcaatgcc tccatgctgg tcagcatgca tggggcccag ctggtcacca ccctcttcct 1681 gccccgtggg gcaactgtgg tagagctctt cccatatgct gtcaatcccg accactacac 1741 tccctataag acgctggcca tgctgcctgg catggacctc cagtatgtag cctggcggaa 1801 catgatgcca gagaacacag tcacacaccc tgagcggccc tgggatcagg ggggcatcac 1861 ccatctggac cgggctgagc aagcccgtat cctgcaaagc cgtgaggtcc cacggcatct 1921 ctgttgccgg aaccccgagt ggctcttccg aatctaccag gacaccaagg tggacatccc 1981 atccctcatt caaaccatac ggcgcgtggt gaagggccgg ccaggaccac ggaagcagaa 2041 gtggacagtc ggcctatatc caggcaaggt gcgggaggca cggtgccagg cgtcagtgca 2101 tggcgcctcc gaggcccgcc tcactgtctc ctggcagatc ccatggaacc ttaaatacct 2161 gaaggtgagg gaggtgaagt acgaggtgtg gctgcaggag cagggggaga acacctacgt 2221 gccttacatc ctggctctgc agaaccacac cttcactgag aacatcaagc ccttcaccac 2281 ctacctggtg tgggtccgct gcatcttcaa caagatcctc ctgggaccct ttgcagatgt 2341 gctggtgtgc aacacgTAGG ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 2401 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG 2461 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA 2521 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC 2581 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT 2641 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA 2701 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA 2761 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC 2821 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA 2881 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC 2941 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT 3001 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT 3061 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT 3121 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT 3181 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT 3241 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA 3301 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG 3361 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT 3421 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC 3481 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC 3541 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 3601 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 3661 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 3721 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 3781 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 3841 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 3901 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 3961 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 4021 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 4081 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 4141 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 4201 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT //